Significant advances in instrumentation and column technology were made to achieve dramatic increases in resolution, speed and sensitivity in liquid chromatography. For the first time, a holistic approach involving simultaneous innovations in particle technology and instrument design was endeavored to meet and overcome the challenges of the analytical laboratory. This was done in order to make analytical scientists more successful and businesses more profitable and productive.
For more than four decades, reducing stationary-phase particle size has been exploited to improve chromatographic separation efficiency. Until recently, LC technology had reached a plateau in which the benefits of reducing particle size could not be fully realized due to the negative influence of instrument band spreading and limited pressure range
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